Table 1.
Abiotic stress confrontation assays.
| Strain-ID | Species | Origin | Drought | H2O2 (μmol/ml) | NaCl (24 h) | NaCl (48 h) | NaCl (72 h) | NaCl (6 days) | OD600 (ONC) | CFU/ml priming suspension | ΔRFW (%) | ΔSFW (%) | ΔGR |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Soil-I-11 | Bacillus sp. | Soil | Tolerant | 0 | 0 | 0 | 8% | 6% | 1.8 | 1013 | 67 | 46 | + |
| Soil-I-14 | Bacillus sp. | Soil | Tolerant | 2,000 | 5% | 7% | 7% | 7% | 1.6 | 4.2*1014 | 83 | 6 | + |
| Soil-I-45 | Sphingomonas sp. | Soil | Tolerant | 0 | 0 | 0 | 8% | 11% | 2.3 | 3.2*1014 | −11 | 11 | + |
| ORE-30 | Sphingomonas sp. | Okra root | Tolerant | 100 | 0 | 0 | 8% | 10% | 2.6 | >3*1014 | 0 | −24 | + |
| ORE-44 | Sphingomonas sp. | Okra root | Tolerant | 0 | 0 | 0 | 8% | 11% | 2.8 | >3*1014 | 21 | −10 | 0 |
| NSRE-37 | Bacillus sp. | Nightshade root | Tolerant | 900 | 0 | 0 | 0 | 0 | 1.7 | 5*1012 | 88 | 56 | + |
Growth after desiccation was measured by CFU/ml: tolerant: CFU above 105 after drought for 88 days. Reactive oxygen species test performed with hydrogen peroxide (H2O2): highest concentration of H2O2, the isolate could still tolerate. NaCl(x): maximum concentration (w/v) of NaCl in growth medium resulting in increased OD600 relative to the control after time x. Increase in root fresh weight (ΔRFW), shoot fresh weight (ΔSFW), and germination rate (ΔGR; +, increase; −, decrease; 0, no effect) relative to the corresponding control group.