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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Cell Metab. 2020 Nov 4;32(6):1012–1027.e7. doi: 10.1016/j.cmet.2020.10.010

Figure 2. Liver-specific TBK1 deficiency increases hepatic lipid accumulation in response to fasting.

Figure 2.

(A~F) NCD-fed flox mice and LTKO mice. (A) Tbk1 mRNA expression in liver tissues from flox or LTKO mice. n = 5–6 mice per group. ***p < 0.001. (Student’s t test). (B) Body weight of 16 week-old NCD-fed mice. n = 11 mice per group. (C) Inflammatory gene expression in liver tissues. n = 4–7 mice per group. (D) Representative images of H&E-stained livers from flox and LTKO mice in ad libitum-fed or 16 h fasted. Scale bar, 50 μm. (E) Hepatic triglyceride (TG) content (mg/g tissue) of NCD-fed mice. n = 5–6 mice per group. (F) Hepatic cholesterol content (mg/g tissue) of NCD-fed mice. n = 6 mice for each group. (G~L) 8 weeks of HFD-fed flox and LTKO mice. (G) Body weight change during HFD feeding. n = 6–9 mice per group. (H) Liver weight of HFD-fed flox and LTKO mice in ad libitum-fed or 16 h fasted. n = 5–7 mice per group. (I) Inflammatory gene expression in liver tissues. n = 6–8 mice per group. (J) Representative images of H&E-stained livers from HFD-fed flox and LTKO mice in ad libitum-fed or 16 h fasted. Scale bars, 100 μm. (K) Hepatic triglyceride (TG) content (mg/g tissue) of HFD-fed mice. n = 6–9 mice per group. (L) Hepatic cholesterol content (mg/g tissue) of HFD-fed mice. n = 6–9 mice per group. For data in (C), (E), (F), (H), (I), (K), and (L), * p < 0.05, **p < 0.01, ***p < 0.001 for flox compared to LTKO in same feeding condition and #p < 0.05, ##p < 0.01, ###p < 0.001 for fed compared to fasted group in same genotype (two-way ANOVA followed Holm-Sidak’s multiple comparisons test). All data in the figure are shown as the mean ± SEM. See also Figure S3.