Figure 4. LTKO mice have increased mitochondria number and size.
(A) The mitochondrial DNA copy number in livers from NCD-fed flox and LTKO mice after 16 h fasting. n = 5–6 mice per group. (B) The mitochondrial DNA copy number in livers from HFD-fed flox and LTKO mice after 16 h fasting. n = 5–6 mice per group. (C) The mRNA levels of mitochondrial biogenesis-related genes from NCD-fed flox and LTKO mice in 16 h fasting. n = 6–7 mice per group. (D) The mRNA levels of mitochondrial biogenesis-related genes from HFD-fed flox and LTKO mice in 16 h fasting. n = 5–6 mice per group. (E) TEM photographs of liver tissues from HFD-fed flox and LTKO mice after 16 h fasting. Scale bar, 2 μm. KC, Kupffer cell; LD, lipid droplet; Mc, mitochondria; Nuc, nucleus; SD, space of Disse or perisinusoidal space; SS, sinusoidal space. (F) Distribution of mitochondrial area. (G) Distribution of mitochondrial length. (H) Mitochondria number per area. For the data in (A), (B), (C), (D), and (H) *p < 0.05, **p < 0.01 (Student t test). For (F) and (G), Kolmogorov-Smimov test was analyzed. N.S., no significance. All data in the figure are shown as the mean ± SEM.
