FIGURE 4.

Adhesion of E. coli Hu 734 on intestinal epithelial cell layers in the absence or presence of adhering B. breve ATCC 15700, B. longum ATCC 15707, B. infantis ATCC 15697 or their adsorbed biosurfactants. E. coli adhesion was enumerated 2 h after initiating E. coli adhesion (before the TEER maximum occurred) and at the TEER maximum (4 h after initiating E. coli adhesion). PBS was used as a control. Error bars represent standard errors of the mean over three experiments with separately grown cellular layers and bacteria. *indicates statistically significant difference in CFUs before the occurrence of TEER maximum and at the TEER maximum.