Fig. 5.

Microcirculatory measurements during wound healing. (a) Ear wounds were generated in a dermal full-thickness mouse model, gas plasma-treated (pl-3 s), and compared to controls (ctrl). Representative false-color images for each microcirculatory parameter on d0, d6, and d15. (b) Microcirculatory wound healing parameters were observed over 15 days using the TIVITA hyperspectral imaging camera system. The tissue oxygenation (StO2 in %), perfusion (NIR), tissue hemoglobin index (THI), and tissue water index (TWI) were measured every 3rd day after wounding in control mice and compared to unwounded tissue (orange). Quantification of characteristic spectral values for each microcirculatory parameter from 500 to 1000 nm after gas plasma treatment (light blue: before; blue: after gas plasma treatment) compared to untreated controls (white). Statistical analysis was performed using one-way ANOVA between gas plasma-treated and untreated mice (n = 4) with *p < 0.05, **p < 0.01, ***p < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)