Table 4.
Primer sequences and restriction endonucleases for determining polymorphic variants of cytochrome and VDR genes.
| Gen | Variant | Primer sequence | Amplified fragment length (bp) | Resriction endonuclease | Products of restriction (bp) |
|---|---|---|---|---|---|
| CYP2C9 | CYP2C9*3 (1075A>C; I359L) |
F 5′ TGCACGAGGTCCAGAGATAT R 5′ACCCGGTGATGGTAGAGGTT |
183 | EcoRV | 1075A−183; 1075C−165+18 |
| CYP3A4 | CYP3A4*3 (c.1334T>C; M445T) | F 5′ GGACACATCACCACCCTGAAT R 5′ TGTTCAGGAGAGCAAACCTC |
395 | FaeI | 1334T−233+139+23; 1334C−233+162 |
| CYP2C9 | CYP2C9*2 (c.430C>T; R144C) |
F 5′ GCATGTGCCTGTTTCAGCAT R 5′ TATGGCCACCCCTGAAATGT |
400 | AspS9I | 430C−81+177+142; 430T−81+319 |
| CYP2D6 | CYP2D6*4 (c.1846G>A) |
F 5′ AGAAGGGCACAAAGCGGGAA R 5′ AGAGACTCCTCGGTCTCTCGC |
264 | MvaI | 1846G−73+191; 1846A−264 |
| CYP3A4 | CYP3A4 *1B (c.-392C>T) | F 5′CAGCCATAGAGACAAGGGCC R 5′ACACACACCACTCACTGACC |
173 | MspI | −392C−19+154; −392T−173 |
| VDR | c.2T>C (FokI) | F 5′ GCCAGCTATGTAGGGCGAAT R 5′ AGGAGGGCTCACCTGAAGAA |
368 | FokI | 2T−131+28+63+146; 2C−131+28+209 |
| VDR | c.1056T>C (TaqI) | F 5′ CTGAGAGCTCCTGTGCCTTC R 5′ ACAAGGGGCGTTAGCTTCAT |
271 | TaqI | 1056T−271; 1056C−73+198 |
| VDR | c.1024+283G>A (BsmI) | F 5′ CCTGAAGGGAGACGTAGCAA R 5′ CACTGCACATTGCCTCCAAA |
351 | BsmlI | 1024+283G−198+153; 1024+283A−351 |