REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-VSV-G tag antibody	Abcam	Cat#ab34774; RRID: AB_778903
Bacterial and Virus Strains
XL10-Gold® Ultracompetent Cells	Agilent Technologies, Inc	Cat#200314
Chemicals, Peptides, and Recombinant Proteins
Collagen from calf skin	Sigma-Aldrich	Cat#C8919; CAS: 9007-34-5
EZ-Link Sulfo-NHS-Biotin	Thermo Scientific	Cat#21217; CAS: 119616-38-5
Nocodazole	Sigma-Aldrich	Cat#M1404; CAS: 31430-18-9
Nvoc-TMP-Cl photocaged chemical dimerizer	Chen et al., 2017	N/A
Poly-L-lysine-hydrobromide	Sigma-Aldrich	Cat#1274; CAS: 25988-63-0
Streptavidin	Serva	Cat#35490.02; CAS: 9013-20-1
Streptavidin Alexa Fluor 750	Life Technologies	Cat#S-21384
Experimental Models: Cell Lines
Human U2OS osteosarcoma cells	ATCC	ATCC HTB-96; RRID: CVCL_0042
Recombinant DNA
delCMV-mCitrine-Rhotekin-GBD	This paper	N/A
mCherry-GEF-H1 C53R	This paper	N/A
mCherry-GEF-H1 PH	This paper	N/A
mCitrine-GEF-H1 PH F539A-I541E	This paper	N/A
mTurquoise2-NES-eDHFR-RhoA Q63L ΔCAAX	This paper	N/A
mTurquoise2-NES-eDHFR-RhoA F30L ΔCAAX	This paper	N/A
mTurquoise2-NES-eDHFR-RhoA wt ΔCAAX	This paper	N/A
mTurquoise2-NES-eDHFR-RhoA T19N ΔCAAX	This paper	N/A
mCherry-Zdk1-GEF-H1 C53R	This paper	N/A
mTurquoise2-eDHFR-GEF-H1 (C53R)	This paper	N/A
mTurquoise2-eDHFR-LARG	This paper	N/A
pTriEx-NTOM20-CCL-moxBFP-CCL-LOV2	This paper	N/A
delCMV-mCherry-Rhotekin-GBD	Graessl et al., 2017	N/A
eDHFR-mCitrine	Chen et al., 2017	N/A
TagBFP-Halotag-CAAX	Chen et al., 2017	N/A
VSVG HaloTag-PARC	Chen et al., 2017	N/A
pTriEx-NTOM20-LOV2	Wang et al., 2016	Addgene Plasmid #81009
pTriEx-mCherry-Zdk1	Wang et al., 2016	Addgene Plasmid #81057
pTriEx-NTOM20-mVenus-LOV2	Wang et al., 2016; Kind gift of Klaus Hahn	N/A
mCitrine-N1	Griesbeck et al., 2001	Addgene Plasmid #54594
mCherry-C1	Clontech	Cat# 632524
moxBFP	Costantini et al., 2015	Addgene Plasmid #68064
EGFP-NMHCIIA	Wei and Adelstein, 2000	Addgene Plasmid #11347
mCherry-NMHCIIA	Dulyaninova et al., 2007	Addgene Plasmid #35687
EGFP-2xFKPB’-Rac1 Q61L ΔCAAX	Liu et al., 2014	N/A
mCherry-LARG	Kind gift of Oliver Rocks	N/A
EGFP-GEF-H1 C53R	Krendel et al., 2002	N/A
pmTurquoise2-NES	Goedhart et al., 2012	Addgene Plasmid #36206
pcDNA3-EGFP-RhoAQ63L	Subauste et al., 2000; Kind gift of Gary Bokoch	Addgene Plasmid #12968
Software and Algorithms
MATLAB R2018	MathWorks	https://de.mathworks.com
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/
Fiji	Schindelin et al., 2012	https://imagej.net/Fiji
Graphpad Prism 7.04 for Windows	GraphPad Software, Inc	https://www.graphpad.com
XPPauto 7.0		http://www.math.pitt.edu/∼bard/xpp/xpp.html
Parameter fitting and posterior distribution analysis scripts	This paper	GitHub;https://github.com/ecam85/optogenetics
custom code for bifurcation analysis, ODE, SDE and CA simulations	This paper	GitHub;https://github.com/agdehmelt/optogenetic_tuning
Cellular automata algorithm	Schmick et al., 2014	N/A