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. 2020 Dec 2;11(12):1028. doi: 10.1038/s41419-020-03200-7

Fig. 6. TAZ silencing promoted T cell infiltration in SKIL-overexpressed tumors through activation of STING pathway.

Fig. 6

M109 cell line with SKIL overexpression, with both SKIL overexpression and TAZ silencing, or control M109 mice lung cancer cell line were injected subcutaneously into BALB/c mice. On day 21, mice were euthanized and tumor blocks were collected. a, b Flow cytometry showed lower number of total T cells (CD45+CD3+) in SKIL-overexpressed tumor compared to control group. Tumors with both SKIL overexpression and TAZ silencing showed significantly higher number of total T cells, compared to tumors with SKIL overexpression. c, d Flow cytometry analysis showed decreased cytotoxic T cells (CD4+CD8+) in tumors with SKIL overexpression, compared to control. Further silencing of TAZ in those cells significantly increased numbers of cytotoxic T cells in those tumors, compared to tumors with SKIL overexpression. e SKIL-overexpressed tumors showed lower levels of CXCL10, CCL5, and IFN-β compared to control. Tumors with both SKIL-overexpression and TAZ silencing showed significantly higher levels of CXCL10, CCL5, and IFN-β, compared to SKIL-overexpression group. f Expression levels of STING, p-TBK1, and p-IRF3 were decreased in SKIL-overexpressed tumors compared to control tumor. Tumors with both SKIL overexpression and TAZ silencing showed significantly higher STING expression and levels of p-TBK1 and p-IRF3 compared to tumors with SKIL overexpression only. *P < 0.05, **P < 0.01. Experiments were performed in triplicate.