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. 2020 Dec 2;11:6179. doi: 10.1038/s41467-020-19884-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic of the NPC assembly and classification of yeast nups. Each subcomplex or type of nup is listed together in a box and the relative position within the NPC assembly is indicated. b Schematic of the Y complex and Nic96 used for alpaca immunization, selection by phage display, and resulting nanobody library. Elements of ACE1 fold proteins are indicated: T—tail and C—crown adjacent to the central trunk element. c Sequence alignment of the nanobody library, colored by percentage identity. Variable complementarity determining regions (CDRs) are indicated.