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. 2020 Dec 2;11:6179. doi: 10.1038/s41467-020-19884-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Spot assays for nanobody and nanobody-mKate2 expression. Strain construction is indicated at the top of the figure, along with assay temperature. Pictures were taken after 48 h on 2% galactose containing agar media. Mock specifies mKate2 expression alone. b Fluorescence images of cells expressing endogenously tagged Nup120-GFP and plasmid expressed NUP120-VHH-mKate2 in DIC, GFP, and far-red channels with a merged overlay. Target of each nanobody is listed in brackets. Scale bar: 5 μM. c Percent of cells with VHH-mKate2 exhibiting nuclear rim enrichment, n = 50 cells per strain over one experiment.