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. 2020 Dec 2;11:6179. doi: 10.1038/s41467-020-19884-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Top and b side view of the cryo-ET map from S. cerevisiae (EMD-10198) with the density for corresponding to the cytoplasmic ring of the NPC colored in light blue. A top-scoring global fit for the composite S. cerevisiae Y complex model shown in both a top and side view. Y complex nups colored as indicated in the legend (right). Nanobodies were not included during global fitting, nanobody-nup complexes were superposed after docking. Nanobodies are shown with surface rendering and labeled with matching colors. Density corresponding to the Nup82 complex is indicated. The position of the Nup133 ALPS motif adjacent to the nuclear envelope is also indicated. c Top and d side view cryo-ET map with the density corresponding to the nucleoplasmic ring of the NPC colored in light green. Top-scoring global fit for the composite S. cerevisiae Y complex model shown in both a top and side view. Nanobody positioning, coloring, and labeling as in a, b.