Table 2.
Data collection and refinement statistics.
| Protein | Nic96189-839, VHH-SAN12 | Nup1201-757, VHH-SAN10, VHH-SAN11 | Nup851-564-Seh1, VHH-SAN2 |
|---|---|---|---|
| PDB code | 6X07 | 6X06 | 6X08 |
| Organism | S. cerevisiae, V. pacos | ||
| Data collection | |||
| Space group | P 21 21 21 | P 61 | P 64 2 2 |
| Cell dimensions | |||
| a, b, c (Å) | 48.1, 79.5, 283.3 | 193.2, 193.2, 78.1 | 234.0, 234.0, 139.4 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 120 | 90, 90, 120 |
| Resolution (Å) | 60.9–2.1 (2.2–2.1)a | 83.7–4.3 (4.4–4.3) | 117.0–4.2 (4.4–4.2) |
| Rp.i.m. (%) | 3.8 (67.7) | 5.2 (51.2) | 5.6 (66.5) |
| I/σ | 23.9 (1.2) | 18.7 (1.4) | 19.1 (1.3) |
| CC1/2 | 1 (0.69) | 0.94 (0.52) | 0.98 (0.64) |
| Completeness (%) | 99.4 (98.9) | 99.5 (99.2) | 99.9 (99.9) |
| Redundancy | 7.2 (6.9) | 7.3 (7.2) | 37.9 (39.9) |
| Refinement | |||
| Resolution range (Å) | 60.9-2.1 | 83.7–4.3 | 117.0–4.2 |
| No. reflections | 64,310 | 11,641 | 16,995 |
| RWork/RFree | 21.6/25.8 | 33.6/35.9 | 31.3/34.7 |
| No. atoms | |||
| Protein | 5,786 | 3818 | 6890 |
| Water | 140 | 0 | 0 |
| B factors (Å2) | |||
| Protein | 88.6 | 241.0 | 207.3 |
| Water | 69.4 | – | – |
| r.m.s. deviations | |||
| Bond length (Å) | 0.011 | 0.002 | 0.006 |
| Bond angles (°) | 1.101 | 0.605 | 0.949 |
aValues in parenthesis are for the highest-resolution shell (10% of the data). One crystal was used for each dataset.