Fig. 2. Sphingolipid ExM enables super-resolution imaging of cellular membranes and protein interactions.

a Confocal fluorescence image of a 10x expanded HeLa229 cell fed with ATTO643-mCling (red) and α-NH₂-ω-N₃-C₆-ceramide clicked with DBCO-Alexa Fluor 488 (green). Scale bars, 20 µm. The images at the bottom show magnified views of the regions outlined by the white boxes in the main images. b Confocal fluorescence image of 4x expanded HeLa229 cells. Cells were fed with α-NH₂-ω-N₃-C₆-ceramide, fixed, permeabilized, and labeled with DBCO-Alexa Fluor 488 (green). In addition, Prx3 (magenta), which is located in the mitochondrial matrix was stained by immunolabeling using ATTO 647 N labeled secondary antibodies. The data were obtained from n = 3 independent experiments. Scale bars, 5 µm.