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. 2020 Dec 2;10:20992. doi: 10.1038/s41598-020-77168-x

Figure 3.

Figure 3

Adhesion kinetics of S. aureus on precoated CVC surfaces. Individual exponential growth phase cells of S. aureus strains N315 and HOM433 were used for SCSF on naïve and pretreated CVC surfaces. (A) Impact of a PBS preincubation (30 min) on the maximum adhesion forces of N315 cells on the surfaces of CVC types I to III. (B) Impact of a HBP preconditioning (30 min) on the maximum adhesion forces of N315 on the surfaces of CVC types I–III. (C) Impact of a HBP pretreatment (30 min) on the maximum adhesion forces of HOM433 on the surface of CVC type III. (D) Maximum adhesion forces of untreated N315 or HOM433 cells on the surface of freshly explanted CVCs of types I and II obtained from patients without CRBSI. (E) Impact of a HBP preconditioning of N315 cells on the maximum adhesion force of the bacterium formed on HBP-precoated surfaces of CVC type III. Data are presented as box and whisker plots (min-to-max) of 40–60 values obtained with 4–6 individual cells per condition. The detection limit of our system is indicated by horizontal dashed lines. *P < 0.05; **P < 0.01 (Mann Whitney U test).