Fig. 2.

Examples of previously-published 2D adipose/breast tumor cell culture systems. A Transwell migration assay to measure tumor cell migration towards adipocyte-secreted chemoattractants. B Using adipocyteconditioned media for modulation of invasiveness in a scratch-wound assay or modulation of other phenotypic markers of breast tumor cells in 2D culture. C Testing the effects of tumor cell-secreted factors on adipocytes using tumor cell-conditioned media in a 2D adipocyte differentiation assay