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. 2020 Dec 2;15:221. doi: 10.1186/s11671-020-03432-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — M1 macrophage exosomal miR-326 promotes apoptosis of HCC cells. a, b Flow cytometry detected the effect of transfection of miR-326 mimic on HepG2 cell cycle; c, d flow cytometry detected the effect of transfection of miR-326 mimic on HepG2 cell apoptosis; e, f flow cytometry detected the effect of transfection of miR-326 inhibitor on SMMC-7721 cell cycle; g, h flow cytometry detected the effect of transfection of miR-326 inhibitor on SMMC-7721 cell apoptosis; i, j flow cytometry detected the effects of co-culture of with miR-326 mimic-transfected M1 macrophage-derived exosomes on HepG2 cell cycle; k, l flow cytometry detected the effect of co-culture with miR-326 mimic-transfected M1 macrophage-derived exosomes on HepG2 cell apoptosis; m, n flow cytometry detected the effect of co-culture with miR-326 inhibitor-transfected M1 macrophage-derived exosomes on SMMC-7721 cell cycle; o, p, flow cytometry detected the effect of co-culture with miR-326 inhibitor-transfected M1 macrophage-derived exosomes on SMMC-7721 cell apoptosis. In panel b and d, *P < 0.05 versus the NC-mimic group; In panel f and h, *P < 0.05 versus the NC-inhibitor group; In panel j and l, *P < 0.05 versus the control group, #P < 0.05 versus the Exo-NC-mimic group; In panel n and p, *P < 0.05 versus the control group, #P < 0.05 versus the Exo-NC-inhibitor group . Measurement data were depicted as mean ± standard deviation (N = 3), comparisons among multiple groups were conducted by one-way analysis of variance