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. 2020 Nov 19;11:587448. doi: 10.3389/fphys.2020.587448

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Copyright © 2020 Wang, Hu, Yao, Zhu, He, Lu, Qi, Xu, Ding, Li, Li and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of TUNAR on human embryo implantation. (A) JAR cells were trypsinized and shaked for 24 h to obtain spheroids of 100–200 μm in size. (B) The spheroids were put onto an human endometrial epithelial cell line (HEEC) monolayer for an hour and the number of spheroids attached was determined as a percentage of the number of spheroids added. (C) A schematic view of the pZW1-snoVector-TUNAR. (D) Expression of TUNAR in HEEC after TUNAR overexpression for 48 h (n = 3). (E) Changes in JAR spheroid attachment rate (attach to HEEC) after TUNAR overexpression for 48 h (n = 3). (F) Expression of TUNAR in Ishikawa cells after TUNAR overexpression for 48 h (n = 3). (G) Changes in JAR spheroid attachment rate (attach to Ishikawa) after TUNAR overexpression for 48 h (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.