Figure 7.

Inoculation of SPZ in Mdr2^−/− mice results in a CD8⁺ and CD4⁺ effector, central, and tissue resident memory T cell expansion. Six-week-old Female Mdr2^−/− naïve mice, primed mice which received one single dose of 10⁴ SPZ, and two challenged groups of mice which received two additional injections of 10⁴ (group 1) and 10⁵ SPZ (group 2) respectively, at 10 days interval (same protocol as in Supplementary Results: Figure 3 ), were sacrificed 90 days following the initial infection with PbANKA parasites (n = 6 per group). Among total liver leukocytes, CD8⁺ and CD4⁺ effector and central memory T cells were stained with the following markers: CD45, CD3, CD8, CD44, CD69, CD62L, and KLRG1. (A) Gating strategy for CD8 Tem (CD3⁺ CD8⁺ CD44^hi CD69⁻ CD62L⁻), Tcm (CD3⁺ CD8⁺ CD44^hi CD69⁻ CD62L⁺), and Trm (CD3⁺ CD8⁺ CD44^hi CD69⁺ CD62L⁻ KLRG1⁻) among CD45⁺ cells. (B) Gating strategy for CD4 Tem (CD3⁺ CD4⁺ CD44^hi CD69⁻ CD62L⁻) Tcm (CD3⁺ CD4⁺ CD44^hi CD69⁻ CD62L⁺), and Trm (CD3⁺ CD4⁺ CD44^hi CD69⁺ CD62L⁻ KLRG1^-) among CD45⁺ cells. Counts of CD3⁺ CD8⁺ cells (C) and CD3⁺ CD4⁺ cells (D) analyzed by flow cytometry expressed as percentage (c–e, and k–o) of CD45⁺ cells and in absolute number of cells (f–j, and p–t). Counts of CD8⁺ CD44^hi cells (C) and CD4⁺ CD44^hi cells (D), analyzed by flow cytometry, were expressed as percentage (C b, D i) and absolute number of cells (C g, D q) of CD3⁺ CD8⁺ or CD3⁺ CD4⁺, respectively. Counts of CD69⁻ CD62L⁻, and CD69⁻ CD62L⁺ cells analyzed by flow cytometry expressed as percentage (c, d, and m, n) and absolute number (h, i, and r, s) of cells of CD3⁺ CD8⁺ CD44^hi cells (C) or CD3⁺ CD4⁺ CD44^hi (D). Counts of CD69⁺ CD62L⁻ KLRG1^- cells analyzed by flow cytometry expressed in percentage (e, o) and absolute number (j, t) of cells of CD3⁺ CD8⁺ CD44^hi (C) or CD3⁺ CD4⁺ CD44^hi cells (D). The asterisks indicate significant differences using the Mann–Whitney test (*p < 0.05; **p < 0.01). Shown data are representative of three independent experiments.