KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal RNA Polymerase II antibody (8WG16)	Neoclone	Cat#WP011
Chemicals, Peptides, and Recombinant Proteins
3-Aminopropyltriethoxysilane	Sigma-Aldrich	Cat#440140 or Cat#A3648
Amine-reactive PEG, 5000 Da	Laysan Bio.	Cat#MPEG-SVA-5000
4-arm Star PEG succinimidyl succinate (Mn~10000 Da)	Laysan Bio.	Cat#4arm-PEG-SC-10K
Amine-reactive biotin-PEG, 5000 Da	Laysan Bio.	Cat#BIOTIN-PEG-SVA-5000
Experimental Models: Cell Lines
HeLa S3	ATCC	Cat#CL-2.2
Experimental Models: Microbes
E. coli BL21-Codon-Plus(DE3)-RP	Agilent	Cat#230255
E. coli BL21-Codon-Plus(DE3)-RIL	Agilent	Cat#230245
E. coli BL21(DE3)pLysS	EMD Millipore	Cat#70236
Oligonucleotides
AdMLP Transcription template no bubble, Top strand: 5’- /5BioTEG/GAA GGG GGG CTA TAA AAG GGG GTG GGG GCG CGT TCG TCC TCA CTC TCT TCC TCA TCA CTT TCT CCT ACC TCC CGG GCT GTT GGC TGC −3’	This paper	N/A
AdMLP Transcription template −14/−3 bubble, Top strand: 5’- /5BioTEG/GAA GGG GGG CTA TAA AAG GGG GTG GGG CAT GCA AGT AGA TCA CTC TCT TCC TCA TCA CTT TCT CCT ACC TCC CGG GCT GTT GGC TGC −3’	This paper	N/A
AdMLP Transcription template −14/−3 bubble no TATA-box, Top strand: 5’- /5BioTEG/GAA GGG GGG CCT GCG GGC GGG GTG GGG CAT GCA AGT AGA TCA CTC TCT TCC TCA TCA CTT TCT CCT ACC TCC CGG GCT GTT GGC TGC −3’	This paper	N/A
AdMLP Transcription template, Bottom strand: 5’- /5Cy5/GCA GCC AAC AGC CCG GGA GGT AGG AGA AAG TGA TGA GGA AGA GAG TGA GGA CGA ACG CGC CCC CAC CCC CTT TTA TAG CCC CCC TTC –3’	This paper	N/A
AdMLP Transcription template no TATA-box, Bottom strand: 5’- /5Cy5/GCA GCC AAC AGC CCG GGA GGT AGG AGA AAG TGA TGA GGA AGA GAG TGA GGA CGA ACG CGC CCC CAC CCC GCC CGC AGG CCC CCC TTC –3’	This paper	N/A
mRNA detection oligo, 5’Cy3: 5’- /5Cy3/GAT GAG GAA GAG AGT −3’	This paper	N/A
mRNA detection oligo, scrambled, 5’Cy3: 5’- /5Cy3/AAA GGT AAG GAT GGG −3’	This paper	N/A
Recombinant DNA
pET-19b hTBP C262T KKCK	This paper	N/A
pET-11c KKCK-hTFIIB	This paper	N/A
pJL2 Gal4-VP16 F150C	This paper	N/A
pET-11c hTFIIγ	(Ozer et al., 1994)	N/A
pET-19b hTFIIAαβ	(DeJong and Roeder, 1993)	N/A
pET-11d hRap30	(Wang et al., 1993)	N/A
pET-23d hRap74	(Wang et al., 1993)	N/A
Software and Algorithms
IDL 6.4	Harris Geospatial Solutions	https://www.harrisgeospatial.com/Software-Technology/IDL
Origin 8.5.0	OriginLab	https://www.originlab.com/index.aspx?go=PRODUCTS/Origin

DeJong, J., and Roeder, R.G. (1993). A single cDNA, hTFIIA/alpha, encodes both the p35 and p19 subunits of human TFIIA. Genes Dev 7, 2220–2234.

Ozer, J., Moore, P.A., Bolden, A.H., Lee, A., Rosen, C.A., and Lieberman, P.M. (1994). Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription. Genes Dev 8, 2324–2335.

Wang, B.Q., Kostrub, C.F., Finkelstein, A., and Burton, Z.F. (1993). Production of human RAP30 and RAP74 in bacterial cells. Protein Expr Purif 4, 207–214.