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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Cancer Discov. 2020 Aug 21;10(12):1854–1871. doi: 10.1158/2159-8290.CD-20-0312

Fig. 4. Donor ML NK exhibit polyfunctional responses to leukemia targets ex vivo.

Fig. 4.

(A) Functional assay schema. Briefly, patient PBMC were collected 8 (n=4) or 14 (n=1) days after NK cell infusion. Lymphocytes were isolated, stimulated with K562, and assessed for the indicated markers by mass cytometry. (B) Frequency of donor NK cells producing the indicated protein after K562 stimulation. Data were analyzed using paired t test (parametric) or Wilcoxon (non-parametric). P-values are indicated within the graph. (C) Poly-functional responses from patient’s donor NK cells. Total indicates the frequency of cells producing at least one cytokine/chemokine. (D) Functionality by licensing status is indicated for donor NK Cells. Solid symbols indicate the KIR was licensed in the donor, open symbols indicate the KIR was unlicensed in the donor. All KIR are predicated to be licensed in the patient. The frequency of each single KIR+ subset is indicated for each KIR within each donor (below the graph). These data indicate that unlicensed ML NK cells are functional.