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. 2020 Nov 19;14:592797. doi: 10.3389/fnana.2020.592797

FIGURE 9.

FIGURE 9

Microphotographs of coronal sections through the rostral (A,B) and middle (C–F) regions of the dentate gyrus of control (A,C,E) and tbl mice (B,D,F) to illustrate the immunoreactivity to caspase 3 and the neuronal marker proteins HuC/HuD. Almost all caspase-3 immunoreactive cells are located within the hilus, the subgranular zone and the granule cell layer, while HuC/HuD also labels interneurons located in the molecular layer. In the control DG it is possible to find caspase-3 labeled cells that do not co-express HuC/HuD (A,C, arrows), whereas in the tbl DG, caspase-3 immunoreactive cells also are labeled by the HuC/HuD antibody (B,D, arrows). Quantitative analysis demonstrates that the tbl DG has significantly more caspase-3-HuC/HuD (G, ***p = 1.902245E–12) and cleaved caspase-3-HuC/HuD double labeled neurons than the controls (H, ***p = 5.524E–06). gcl, granule cells layer; h, hilus; mol, molecular layer; sgz, subgranular zone. Bar = 200 μm (A–F), and 30 and 60 μm (F).