. 2020 Dec 1;6(12):e05556. doi: 10.1016/j.heliyon.2020.e05556

Table 2.

Frequency of different subtypes of CD4⁺ Treg cells in tumor draining lymph nodes from patients with bladder cancer.

Cell subset	Phenotype	Min	Max	Median	Mean ± SEM
CD4⁺FOXP3⁺	CD4⁺FOXP3⁺	10.26	69.19	26.03	30.13 ± 2.17
Treg	CD4⁺CD25⁺FOXP3⁺CD127^low/neg	0.05	24.1	9.88	9.92 ± 0.8
CD127⁺ Treg	CD4⁺CD25⁺FOXP3⁺CD127⁺	0.28	16	2.79	3.74 ± 0.43
CD4⁺CD25⁺	CD4⁺CD25⁺	10.77	29.49	19.2	19.63 ± 0.74
CD4⁺CD127⁺	CD4⁺CD127⁺	51.29	99.7	75.93	75.08 ± 2.06
Mean expression of FOXP3 molecule in different CD4+ subsets (based on MFI)
Treg	CD4⁺CD25⁺FOXP3⁺CD127^low/neg	0.79	2.81	1.71	2.03 ± 0.22
CD127⁺ Treg	CD4⁺CD25⁺FOXP3⁺CD127⁺	1.19	4.6	1.55	1.86 ± 0.18
CD4⁺FOXP3⁺	CD4⁺FOXP3⁺	1.22	3.58	1.75	1.95 ± 0.12

MFI: mean fluorescence intensity.