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. 2020 Oct 21;27(5):dsaa024. doi: 10.1093/dnares/dsaa024

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© The Author(s) 2020. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Localization of BG4 binding within HeLa cells in nutrition proficient and deficient conditions. (A) Evaluation of BG4 binding to G4 structures in HeLa under nutrition proficient (presence of FBS) and deficient (absence of FBS, incubated for 48 h) conditions. Immunofluorescence was performed for BG4 as described above. BG4 foci are marked in both nuclear and cytoplasmic regions. Serum starvation of cells for 48 h, followed by BG4 staining, revealed more localization of the antibody towards the cytoplasm. (B) Scatter plot illustrates the comparison of G4 structure formation in nutrition proficient and deficient conditions in HeLa cells.