Figure 5.

circRASGRF2 promotes migration and invasion of HCC cells in vitro and enhances the metastasis of xenograft tumors of HCC cells in vivo

(A) Transwell assays showed that the inhibition of circRASGRF2 expression attenuated cell invasion and migration of MHCC97H cells. (B) Transwell assays showed that the overexpression of circRASGRF2 promoted cell invasion and migration of Hep3B cells. (C) Western blot assays showed that circRASGRF2 knockdown remarkably increased the expression of E-cadherin and decreased the expression of N-cadherin and vimentin in MHCC97H cells. (D) Western blot assays showed that overexpression of circRASGRF2 decreased the expression of E-cadherin and increased the expression of N-cadherin and vimentin in Hep3B cells. (E) Representative images of lung metastasis in mice inoculated with MHCC97H cells for 35 days were taken. (F) circRASGRF2 depletion significantly reduced the lung metastases burden of MHCC97H cells. All tests were at least performed three times. Data are expressed as mean ± SD. ∗∗p < 0.01.