Figure 6.

circRASGRF2 acted as a miR-1224 sponge in HCC cells

(A) Endogenous circRASGRF2 was efficiently pulled down by anti-Ago2. (B) miRNA pull-down assay showed that circRASGRF2 was only efficiently enriched by miR-1224. (C) Binding sequence between miR-1224 and circRASGRF2. (D) The luciferase reporter systems showed that the miR-1224 mimic considerably reduced the luciferase activity of the WT-circRASGRF2 luciferase reporter vector compared with the negative control, while the miR-1224 mimic did not pose any impact on the luciferase activity of MUT-circRASGRF2-transfected MHCC97H cells. (E) circRASGRF2 and miR-1224 simultaneously existed in the production precipitated by anti-AGO2. (F) Silencing of circRASGRF2 did not affect the expression of miR-1224. (G) Transfection of miR-1224 mimics did not affect the expression of circRASGRF2. (H) The level of miR-1224 was significantly decreased in HCC cells compared to L02 cells. (I) The level of miR-1224 was significantly decreased in HCC tissues compared to ANL tissues. (J) miR-1224 could significantly reverse the circRASGRF2 overexpression-mediated promotion of proliferation. All tests were performed at least three times. Data are expressed as mean ± SD. ∗∗p < 0.01.