Fig. 4.

Schema of the steps to identify hotspot modeled as position specific. (A) Mutations are first contextualized relative to covariates. (B) Context is used to estimate specific background probabilities and a statistic representing the observed number of mutations. (C) The statistic is used to estimate a probability value. (D) The p-value and their annotation is considered to report significant hotspots. (E) Selected examples of methods with specific conceptual choices where symbols and colors represent implemented features.