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Other methods to detect hotspots or putatively false regions. (A) Functional impact methods quantify the effect of mutations in protein function, RNA structure, binding sites, or miRNA/lncRNA targets. (B) Estimation of structural features that increase the susceptibility of DNA mutations can be used to mark possible false regions. (C) Summary statistics, such as the distribution of mutated positions, can be used to detect positions that are over mutated.
