Table 2.
Properties of the wildtype and the mutant residues, and their impact on protein structure
| Property | Outcome |
|---|---|
| Residue properties of wildtype and mutant are the same (e.g., aliphatic to aliphatic substitution) | Low Impact |
| Change in residue property of the mutant (e.g., aliphatic to aromatic substitution) | High Impact |
| Conservation score > 0(variable residue) (as measured by ConSurf) | Low Impact |
| Conservation score < 0 (conserved residue) | High Impact |
| Interface Residue = No (more than 5 Å from the subunit interface) | Low Impact |
| If the mutating residue is an interface residue (<5 Å from the subunit interface) | High Impact |
| No change in secondary structure due to mutation (identified using SDM2) | Low Impact |
| Change in secondary structure due to mutation | High Impact |
| If the distance of the mutating residue from the ligand is < 5 Å | High Impact |
| If the distance of the mutating residue from the ligand is >5 Å | Low Impact |
| If the distance of the mutating residue from the nucleic acid is < 5 Å | High Impact |
| If the distance of the mutating residue from the nucleic acid is >5 Å | Low Impact |