Residue properties of wildtype and mutant are the same (e.g., aliphatic to aliphatic substitution) |
Low Impact |
Change in residue property of the mutant (e.g., aliphatic to aromatic substitution) |
High Impact |
Conservation score > 0(variable residue) (as measured by ConSurf) |
Low Impact |
Conservation score < 0 (conserved residue) |
High Impact |
Interface Residue = No (more than 5 Å from the subunit interface) |
Low Impact |
If the mutating residue is an interface residue (<5 Å from the subunit interface) |
High Impact |
No change in secondary structure due to mutation (identified using SDM2) |
Low Impact |
Change in secondary structure due to mutation |
High Impact |
If the distance of the mutating residue from the ligand is < 5 Å |
High Impact |
If the distance of the mutating residue from the ligand is >5 Å |
Low Impact |
If the distance of the mutating residue from the nucleic acid is < 5 Å |
High Impact |
If the distance of the mutating residue from the nucleic acid is >5 Å |
Low Impact |