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. 2020 Dec 3;11:521. doi: 10.1186/s13287-020-02035-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Reduction of MMP activity by ASC-CM, TIMP quantification, and MMP expression. a MMP activity, analyzed in CH culture medium (n = 7 independent experiments) 3 and 6 days after the treatments, is expressed as arbitrary fluorescence units (AFU). All conditions statistically differ from control (at day 3: TNF p < .01, TNF+ASC-CM p < .05, and TNF+ASC-EV p < .001; at day 6: TNF p < .001, TNF+ASC-CM p < .05, and TNF+ASC-EV p < .001). Significance vs 10 ng/ml TNFα is shown as ^$p < .05; vs ASC-EV as ^#p < .05, ##p < .01. b TIMP-1 and 2 data are expressed as label-free quantification (LFQ) intensity, count per second (cps) from differential proteomic analysis of 20 μg of ASC-CM and -EV proteins. Means ± SD (n = 3) are shown. c, d Quantification of the expression of MMP-13 (c) and MMP-3 (d) in TNFα-stimulated and ASC-CM- or -EV-treated CH at day 3 and 6 analyzed by Western blot. Data (n = 5 independent experiments) were normalized on GAPDH and expressed as relative values (CTRL = 1)