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. 2020 Dec 3;11:521. doi: 10.1186/s13287-020-02035-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Hypertrophy and inflammatory markers induced by TNFα treatment. a PGE2 levels, quantified in CH culture medium at day 3 and 6 after treatments, are expressed as ng/ml (n = 6 independent experiments). b COL10A1 expression by Western blot analysis. Data (n = 5 independent experiments) were normalized on GAPDH and shown as relative values (CTRL = 1). Data in a and b were analyzed by Friedman’s test followed by Dunn’s multiple comparison test and significance vs CTRL is shown as *p < .05. For each column, the box extends from the 25th to 75th percentiles, the line in the middle is plotted at the median while the whiskers indicate minimum and maximum value. c CH protein concentration at day 3 and 6 (left and right panel, respectively). Significance vs CTRL is shown as *p < .05 and **p < .01; vs 10 ng/ml TNFα as ^$p < .05 (n = 7 independent experiments). d Cx43 expression by Western blot. Data (n = 5 independent experiments) were normalized on GAPDH and expressed as relative values (CTRL = 1). Significance vs CTRL is shown as *p < .05 and **p < .01