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. 2020 Dec 3;26:120. doi: 10.1186/s10020-020-00248-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Apoptosis was detected via TUNEL assay with a fluorescence microscope (× 200). There was no abnormal apoptosis detected in control group at any of the time points (images a). TUNEL staining showed gradual increases in apoptosis in model group (images b–d). Scale bar = 50 um. The apoptosis index in model group was significantly higher than that in control group at each time point (images e). DAPI wss used to mark all cells, blue dots in the figure, and TUNEL wss used to stain apoptotic cells, green dots in the figure) (*compared with control group, P < 0.05. The red arrow shows the TUNEL-positive cells). Model group vs control group: the 2nd week (n = 9 vs 8), 4th week(n = 8 vs 8), and 6th week (n = 9 vs 8)