Skip to main content
. Author manuscript; available in PMC: 2020 Dec 3.
Published in final edited form as: Methods Cell Biol. 2015 May 27;129:1–18. doi: 10.1016/bs.mcb.2015.03.013

FIGURE 4. Serial sectioning and picking up the sections.

FIGURE 4

(A) The sample in a position ready for sectioning. (B) A knife boat is filled with water before sectioning. (C) Stills from Video 1 illustrating the movement of the trapezoidal prism during sectioning and the formation of the ribbon of sections. (D) The ribbon floating on the water with the last section still attached to the edge of the knife. (E) The long ribbon is separated in four shorter ribbons before the pickup. The ribbons are free-floating in a knife boat filled with water. (F) The coated grid partially submerged in the water in preparation for the ribbon pickup. (G) Examples of three formvar coated grids; the left one is empty, and the middle and the right one carry a ribbon of serial sections.