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. Author manuscript; available in PMC: 2020 Dec 3.
Published in final edited form as: Nat Hum Behav. 2019 Jul 29;3(9):950–961. doi: 10.1038/s41562-019-0653-z

Figure 4. Comparison of Zip8 alcohol phenotypes in Drosophila.

Figure 4.

Flies were exposed to 100/50 Ethanol/Air vapor for 30 min for exposure 1, and the time to 50% loss of righting was determined (ST-50, sedation time). After recovery on food for 4 hours, flies were re-exposed to the same vapors, and the second ST-50 recorded (left side). The resulting increase in ST-50, i.e. tolerance, is shown on the right. In a) overexpressed human hZIP8 in ics-expressing cells flies are compared against controls whereas in b) knockdown of the fly ortholog dZip71B is compared against controls. In c) flies carrying two transposon insertions in the endogenous dZip71B gene are compared against controls. Significance levels: ***P <0.001, **P <0.01, *P <0.05. Exact P-values are presented in the text.