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. 2020 Nov 24;38:101800. doi: 10.1016/j.redox.2020.101800

Fig. 1.

Fig. 1

Kinetic characterization of human GOT1 and GOT2 isoenzymes. A./B. Steady state kinetics of human GOT1 (A) and GOT2 (B) using aspartate as substrate. Activity of purified GOT (1.5 μM) was determined using standard assay conditions and HPLC detection of the reaction product glutamate. Error bars indicate standard deviations (n = 3). C./D. Steady state kinetics of human GOT1 (C) and GOT2 (D) using cysteine sulfinic acid (CSA) as substrate. Activity of purified GOT (1.5 μM) was determined using standard assay conditions and HPLC detection of the reaction product glutamate. Error bars indicate standard deviations (n = 3).