Fig. 3.
Dependence of sulfite production on cellular cysteine- and CDO levels. A. Sulfite measurement from cell extracts of WT, SUOX−/−, GOT1−/−/SUOX−/− and GOT2−/−/SUOX−/− cells after incubation in different cysteine concentrations (n = 4). Sulfite levels were adjusted to cellular protein concentration. Values were normalized to WT. Error bars indicate standard deviation. Two-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05. B. Sulfite measurement from cell extracts of WT, SUOX−/−, GOT1−/−/SUOX−/− and GOT2−/−/SUOX−/− cells following overexpression of CDO (n = 4). Sulfite levels were adjusted to cellular protein concentration. Values were normalized to WT. Error bars indicate standard deviation. Two-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05.