Fig. 4.

Determination of cellular levels of H₂S, persulfidated cysteine and –glutathione in SUOX^−/−cells. A. Putative production of persulfidated cysteine (CysSH) and –glutathione (GSSH). B. Determination of H₂S levels from WT, SUOX^−/−, GOT1^−/−/SUOX^−/− and GOT2^−/−/SUOX^−/− cells. Error bars indicate standard deviation. One-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05. C. Determination of total free cysteine, free cysteine persulfide and the percentage of persulfidated cysteine from WT, SUOX^−/−, GOT1^−/−/SUOX^−/− and GOT2^−/−/SUOX^−/− cells. Error bars indicate standard deviation. One-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05. D. Determination of total glutathione, glutathione persulfide and the percentage of persulfidated glutathione from WT, SUOX^−/−, GOT1^−/−/SUOX^−/− and GOT2^−/−/SUOX^−/− cells. Error bars indicate standard deviation. One-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05.