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. 2020 Nov 24;38:101800. doi: 10.1016/j.redox.2020.101800

Fig. 5.

Fig. 5

Expression levels of proteins involved in H2S metabolism. A. Expression levels of proteins involved in cysteine catabolism (SO, SQR, CBS, CSE, TST, GOT1, MPST, GOT2 and ETHE1) in WT, SUOX−/−, GOT1−/−/SUOX−/− and GOT2−/−/SUOX−/− cells. Tubulin was used as a loading control. B. Quantification of expression changes of SQR in relation to tubulin and WT samples (n = 4). Error bars indicate standard deviation. One-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05.C. Quantification of expression changes of ETHE1 in relation to tubulin and WT samples (n = 4). Error bars indicate standard deviation. One-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05. D. Quantification of expression changes of CSE in relation to tubulin and WT samples (n = 4). Error bars indicate standard deviation. One-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05. E. Quantification of expression changes of CBS in relation to tubulin and WT samples (n = 4). Error bars indicate standard deviation. One-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05. F. Quantification of expression changes of GOT1 in relation to tubulin and WT samples (n = 4). Error bars indicate standard deviation. One-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05. G. Quantification of expression changes of GOT2 in relation to tubulin and WT samples (n = 4). Error bars indicate standard deviation. One-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05. H. Quantification of expression changes of MPST (cytosolic and mitochondrial isoforms) in relation to tubulin and WT samples (n = 4). Error bars indicate standard deviation. One-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: *** <0.001; ** <0.01; * <0.05; ns > 0.05.