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. Author manuscript; available in PMC: 2020 Dec 3.
Published in final edited form as: Biochem J. 2013 Jul 1;453(1):125–136. doi: 10.1042/BJ20130012

Figure 2. NKX3.1 homeodomain is required for binding to the topoisomerase I linker domain.

Figure 2

HEK-293T cells were transfected with Myc–NKX3.1 fusion constructs (A) and GST pull-down assays were performed with GST–topoisomerase I(636–697) (B). Equal amounts of GST–topoisomerase I(636–697) were used in each reaction as indicated by immunoblot (IB) analysis with an anti-GST antibody (B, lower panel).