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. Author manuscript; available in PMC: 2020 Dec 3.
Published in final edited form as: Biochem J. 2013 Jul 1;453(1):125–136. doi: 10.1042/BJ20130012

Figure 3. Effect of NKX3.1 on Topo70-induced DNA relaxation.

Figure 3

(A) Purified Topo70 (1:32–1:1024 dilution) was used in DNA-relaxation assays in the absence or presence of 400 ng of purified NKX3.1 per reaction. (B) Graphical depiction of the DNA-relaxation activity from (A). (C) Topo70 (1:256 diluted) was used in DNA-relaxation assays in the absence or presence of increasing amount of purified NKX3.1 (0–400 ng/reaction). (D) The relative relaxation activities from the results shown in (C).