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. Author manuscript; available in PMC: 2020 Dec 3.
Published in final edited form as: Biochem J. 2013 Jul 1;453(1):125–136. doi: 10.1042/BJ20130012

Figure 5. Binding of NKX3.1 to reconstituted topoisomerase I (TopoI) peptides.

Figure 5

GST or GST–topoisomerase I(201–635) was first loaded on to glutathione–Sepharose 4B beads at room temperature for 30 min and the unbound proteins were removed by washing. Beads were then incubated with purified topoisomerase I(698–765) or topoisomerase I(636–765), in the absence or presence of increasing amounts of NKX3.1. The beads were then washed extensively, subjected to SDS/PAGE (4–20% gel) analysis and the proteins visualized by Coomassie Blue staining.