Figure 5.

Expression of (a) MDA-5 (b) IFN-α (c) IFN-β (d) Mx. genes in Bursa of vaccinated and Non-vaccinated Aseel and White Leghorn chicken infected with very virulent IBDV. The groups were as follows: VAG-Vaccinated Aseel Group; VWG-Vaccinated White Leghorne group; NAG-Non-vaccinated Aseel Group; NWG- Non-vaccinated White Leghorn Group. Error bars represent standard error of the mean. Relative changes in the expression of genes were quantified by real-time PCR by comparison with expression levels of the housekeeping gene GAPDH and expressed as the fold-change. The value of 1.00 was kept as the control and the fold change values which were above the control values were considered as significant. * significant difference comparing VAG with VWG and NAG with NWG chickens of the same line at the same point where p < 0.01 **, p < 0.001 ***. (a–c): Experimental groups sharing common lowercase superscript letters are statistically non-significant for each observation day.