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. 2020 Nov 19;11:567391. doi: 10.3389/fimmu.2020.567391

Figure 1.

Figure 1

Treatment with MV130 modifies biological properties of MSCs. MSCs were treated with MV130 for 2 h. (A, B) After 24 h, MSCs were stained with Annexin V and IP and cell viability was analysed by flow cytometry. Mean ± SEM of 5 independent experiments (A) and a representative experiment (B) are shown. (C) MSCs were treated with MV130 for 2 h. Bcl2 and Bcl-xL expression in MSCs were analyzed by flow cytometry 24 h after treatment. The percentages of positive cells are indicated in each histogram. Gray filled histograms represent isotype control staining. Data are representative of 4 independent experiments. (D) 48 h after treatment, the expression of different surface markers was studied on MSCs by flow cytometry. Representative histograms and MFI values are shown (n = 3-4). Gray histograms represent isotype controls. (E) mRNA expression for different immunomodulatory factors was studied on MSCs by qRT-PCR. Data represent mean ± SEM of 8 to 12 independent experiments relative to individual controls. (F) Supernatants from MSC cultures were collected 48 h after treatment. Protein secretion relative to individual controls is expressed as mean ± SEM from 15 independent experiments (*p < 0.05; **p < 0.01***p < 0.005 by Wilcoxon test).