Skip to main content
. 2020 Nov 19;11:567391. doi: 10.3389/fimmu.2020.567391

Figure 2.

Figure 2

MV130 is uptaken by oral MSCs in vivo. (A) Flow cytometry analysis of T lymphocytes (CD45+CD3+), macrophages (F4/80+MHC-IIlo), DCs (F4/80-MHC-II+) and MSCs (CD29+Sca-1+) present in the oral mucosa from mice after sublingual immunization with MV130-CFSE. Histograms show the percentage of uptake of CFSE-MV130 by each of these populations. Data are representative of 3 independent experiments. (B) Percentage of T cells, granulocytes and MSCs present in peripheral lymph nodes (P-LN), submaxillary lymph nodes (SM-LN) and oral mucosa (OM) from mice after sublingual immunization with MV130 respect to control mice (n = 6–13). (C) MV130 priming reduces MSC migration capacity. Bar graph shows the percentage of migrating cells in MV130 primed cultures. Results represent the mean ± SEM of 3 independent experiments (D) MV130 does not specifically attract MSCs. Bar graph shows the percentage of migrating cells to MV130 or IFNγ. Results represent the mean ± SEM of 4 independent experiments. (*p < 0.05; ***p < 0.005 by Wilcoxon test).