Table 2.

Threshold genomic distances between fungal species and genera for different k-mer sizes calculated with Mash and Dashing programs; commands for calculating the distances are listed in Table 1.

Method	k-mer Size *	Same Species	Genomic Distance Threshold **	Different Species
Mash	14	less than	0.004129	greater than
Mash	16	less than	0.04317	greater than
Mash	18	less than	0.05781	greater than
Mash	20	less than	0.05915	greater than
Mash	22	less than	0.05833	greater than
Dashing	14	greater than	0.9076	less than
Dashing	16	greater than	0.3736	less than
Dashing	18	greater than	0.2437	less than
Dashing	20	greater than	0.2084	less than
Dashing	22	greater than	0.1863	less than

* The accuracy of the classification by thresholds was similar for different k-mer sizes in the table (0.17% difference between the minimum and maximum accuracy in case of Mash distances, and 0.60% difference in accuracy in case of Dashing distances). ** Distances were calculated from a reduced genome dataset of 1319 genomes after excluding incompletely identified species, species hybrids and outlier genomes and species (full description of the dataset is provided in the Methods section), therefore the thresholds in this table are different from the thresholds determined on the full dataset (Figure 2, Figure 3 and Figure 4).