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(A) Differential pulse voltammetry (DPV) curves of Apt/ERGO-GCE in the absence and presence of α-Syn oligomer in 0.1 M TBS. Effect of (B) the accumulation times ([Apt] = 1 μM) and (C) Apt concentration (accumulation time = 40 min) for Apt immobilization on ip in 0.1 M TBS buffer. (D) Effect of incubation time for α-Syn oligomer binding on ip in 0.1 M TBS buffer. [α-Syn oligomer] = 1 nM, [Apt] = 1 μM, Accumulation time = 40 min, Room temperature.
