Table 1.
Cryo‐ET data collection parameters.
| Condition | WT | δ‐84 | δ‐84 | δ‐84 + Munc‐18 |
|---|---|---|---|---|
| Voltage (kV) | 300 | 300 | 300 | 300 |
| Volta phase plate | Yes | Yes | Yes | Yes |
| Detector | Gatan K2 | Gatan K2 | Gatan K2 | Gatan K2 |
| Energy filter slit width (eV) | 20 | 20 | 20 | 20 |
| Electron exposure (e/Å2) | ~ 200 | ~ 200 | ~ 200 | ~ 200 |
| Defocus (μm) | 0 | 0 | 0 | 0 |
| Tilt range (min/max, step) | −60°/+60°, 1° | −60°/+60°, 1° | −60°/+60°, 1° | −60°/+60°, 1° |
| Tilt scheme | Dose‐symmetrical (Hagen Scheme) | Dose‐symmetrical (Hagen Scheme) | Dose‐symmetrical (Hagen Scheme) | Dose‐symmetrical (Hagen Scheme) |
| Tomogram used/acquired | 18/21 | 8/15 | 5/5 | 17/21 |
| Pixel size (Å) | 5.31 | 4.51 | 4.80 | 4.35 |
| Docked vesicles analysed | 155 | 64 | 26 | 133 |