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. 2020 Oct 9;8(4):592. doi: 10.3390/vaccines8040592

Figure 2.

Figure 2

6E8 and 9A10 block PRRSV infection in PAMs. Inhibition in PRRSV infection by 6E8 and 9A10 in PAMs was detected using Western blotting (A), qRT-PCR (B), and virus titration (C). PAMs were preincubated with 50 μg/mL mAb for 1h, followed by the inoculation with PRRSV ZJfh17. Cells were collected at 24 hpi and titrated. β-actin was detected as the internal controls. (D) Virus inhibition with 6E8 and 9A10 at different concentrations in PAMs. 6D10 served as an irrelevant mAb control. (E) Comparison between mAbs (6E8, 9A10, and 9A10+6E8) in ZJfh17 inhibition in PAMs. 9A10 and 6E8 were mixed at a ratio of 50:50 (w/w). PRRSV N expression was used to indicate relative changes of viral mRNA as compared to the infection group without antibody treatment after normalization using GAPDH mRNA. Experiments were performed in triplicate and data were shown as mean ± SD. Statistical significance is indicated as * (p < 0.05); ** (p < 0.01); *** (p < 0.001); **** (p < 0.0001).