Figure 5.

9A10 and 6E8 mAbs suppress CD163 transcription. Total RNA was extracted to evaluate mRNA expression of CD163 in PAMs (A) and Marc-145 (B). Cells were mock treated or treated with mAbs (100 μg/mL) for 1 h, and then cultured for 24 h. (C) MG132 (20 μg/mL) inhibited CD163 transcription in Marc-145. (D) Time-coursed suppression in CD163 transcription with mAbs or MG132. GAPDH was detected as the internal reference. Experiments were performed in triplicate and data are shown as mean ± SD. Statistical significance is indicated as * (p < 0.05); **** (p < 0.0001).