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. 2020 Oct 9;8(4):592. doi: 10.3390/vaccines8040592

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fine mapping of epitopes recognized by 6E8 and 9A10. Schematic diagram of various CD163 SRCR5–9 truncated fragments for epitope mapping with 6E8 (A) and 9A10 (C). SRCR5–9 fragments 1 to 5 and subfragments were expressed in recombinant baculovirus in Sf9 insect cells and screened with individual mAb in IFA. (B,D) IFA with mAbs in insect cells expressing point mutated Ac-CD163 SRCR5–9. Alanine substitutions at aa90-aa101 (B) and aa305-aa331 (D) of Ac-CD163 SRCR5–9 were performed individually.