Table 1.
Name | Forward (5′-3′) | Reverse (5′-3′) | Amplicon (bp) | Target | Assay |
---|---|---|---|---|---|
OPA14F/OPA14R | TCTGTGCTGGCCCTTATCG | TCTGTGCTGGGTACTGACGTG | 455 | Fungus | SCAR |
BbITS4/BbITS5 | TCCTCCGCTTATTGATATGC | GGAAGTAAAAGTCGTAACAAGG | ~600 | Fungus | ITS |
BbEF1T/Bb1567R | ATGGGTAAGGARGACAAGAC | ACHGTRCCRATACCACCSATCTT | ~1200 | Fungus | EF1-α |
qBbtenS | ACTGTCCGCATTGGCAGCTAAG | TGTCCTTTGGTGGTGGTGATGG | 113 | Fungus | qRT-PCR |
qBbbeaS | GTTCTTCCTCCGCATTCCGTTC | TAGAGCGCAACGTCTTTCGGTC | 97 | Fungus | qRT-PCR |
qBbbslS | CAATCGACTGAGACGCCATTCC | TTTGACCTGCGAATCCATACGG | 156 | Fungus | qRT-PCR |
qTi18S | GGCGGGGGCATTCGTATTG | ATCGCTGGCTGGCATCGTTTAT | 123 | Insect | qRT-PCR |
qTiEF1 | AAAGTGCGACCGTCGTACAGG | TCACGAACGGCAAAGCGA | 100 | Insect | qRT-PCR |
qTiLp2 | GCATTTCTGCCAAGAAGAGG | ATGGAATCAAAGCTGGCCTA | 110 | Insect | qRT-PCR |
qTiDef1 | TGACTTAGCCCAGCAACCAT | GCACAGGCTGCATGATTAGG | 150 | Insect | qRT-PCR |
qTiDef2 | CTTCTTAGTAGCCGCCCTCG | GGTGCCACCATCGTATCCAT | 210 | Insect | qRT-PCR |
qTiDef6 | TGAAGTGTGCACTCTCTTTGGT | GGCTCCCATAGGGCTTCATC | 106 | Insect | qRT-PCR |