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. 2020 Oct 12;6(4):219. doi: 10.3390/jof6040219

Table 1.

Oligonucleotides used in this study.

Name Forward (5′-3′) Reverse (5′-3′) Amplicon (bp) Target Assay
OPA14F/OPA14R TCTGTGCTGGCCCTTATCG TCTGTGCTGGGTACTGACGTG 455 Fungus SCAR
BbITS4/BbITS5 TCCTCCGCTTATTGATATGC GGAAGTAAAAGTCGTAACAAGG ~600 Fungus ITS
BbEF1T/Bb1567R ATGGGTAAGGARGACAAGAC ACHGTRCCRATACCACCSATCTT ~1200 Fungus EF1-α
qBbtenS ACTGTCCGCATTGGCAGCTAAG TGTCCTTTGGTGGTGGTGATGG 113 Fungus qRT-PCR
qBbbeaS GTTCTTCCTCCGCATTCCGTTC TAGAGCGCAACGTCTTTCGGTC 97 Fungus qRT-PCR
qBbbslS CAATCGACTGAGACGCCATTCC TTTGACCTGCGAATCCATACGG 156 Fungus qRT-PCR
qTi18S GGCGGGGGCATTCGTATTG ATCGCTGGCTGGCATCGTTTAT 123 Insect qRT-PCR
qTiEF1 AAAGTGCGACCGTCGTACAGG TCACGAACGGCAAAGCGA 100 Insect qRT-PCR
qTiLp2 GCATTTCTGCCAAGAAGAGG ATGGAATCAAAGCTGGCCTA 110 Insect qRT-PCR
qTiDef1 TGACTTAGCCCAGCAACCAT GCACAGGCTGCATGATTAGG 150 Insect qRT-PCR
qTiDef2 CTTCTTAGTAGCCGCCCTCG GGTGCCACCATCGTATCCAT 210 Insect qRT-PCR
qTiDef6 TGAAGTGTGCACTCTCTTTGGT GGCTCCCATAGGGCTTCATC 106 Insect qRT-PCR